New White Paper - A single Snapshot for Whole-Organoid Screening

 

View Online     APPLICATION NOTE Capturing the whole organoid in a single shot     Dear Researcher, If you screen drug response in 3D organoid models, you know the trade-off well. Image each organoid in full 3D and a whole plate can take a very long time; flatten it to a single 2D plane and you lose the very morphology that made a 3D model worth using. We are excited to share an application note we recently published with InSphero that offers a way around this. Using the ZEISS LSM 990 with Lightfield 4D, we captured the entire volume of each organoid in a single snapshot — no serial z-stacking — and ran quantitative drug-response screening across a full 384-well plate.     A few results stood out. Colorectal tumor–stroma co-cultures treated with the topoisomerase inhibitor SN-38 showed a clear, dose-dependent loss of tumor cells — roughly 57% at the highest dose — while the surrounding stroma held steady, pointing to a tumor-directed effect. And because the whole volume is already captured, we could analyze the same data in true 3D and recover 2–2.5× more cells than a 2D projection would, with no cost to throughput. Wells worth a closer look were simply re-imaged with Airyscan on the same instrument — no sample transfer needed.        1-Snap Full organoid volume,  no z-stepping.     ~57% Tumor-cell loss at 500 nM SN-38.     2-2.5x More cells counted in 3D vs. 2D.     It’s a short read, about nine minutes. If any of this is close to your work, I think you’ll find it worthwhile:     Read the Application Note     Download the full White Paper     Best regards,  Your ZEISS Team This work was carried out in collaboration with InSphero, whose Akura™ 384 ImagePro platform and reproducible tumor–stroma organoid co-cultures formed the biological basis of the study.

You can find more information on: ZEISS Website  →     Carl Zeiss Microscopy LLC One North Broadway White Plains, New York 10601 United States Phone: +1 800 233-2343 microscopy@zeiss.com Commercial register: Jena, HRB 210536 VAT ID No. DE814503774 Chairman of the Supervisory Board: Dr. Marc Wawerla Management Board: Sandro Förster, Dr. Florian Mezger